KMID : 0043319980210060712
|
|
Archives of Pharmacal Research 1998 Volume.21 No. 6 p.712 ~ p.717
|
|
Nucleotide and Deduced Amino Acid Sequences of Rat Myosin Binding Protein H (MyBP-H)
|
|
Jung Jae-Hoon
Oh Ji-Hyun Lee Kyung-Lim
|
|
Abstract
|
|
|
The complete nucleotide sequence of the cDNA clone encoding rat skeletal muscle myosin- binding protein H (MyBP-H) was determined and amino acid sequence was deduced from the nucleotide sequence (GenBank accession number AF077338). The full-length cDNA of 1782 base pairs(bp) contains a single open reading frame of 1454 bp encoding a rat MyBP-H protein of the predicted molecular mass 52.7kDa and includes the common consensus 1CA__TG¡¯ protein binding motif. The cDNA sequence of rat MyBP-H show 92%, 84% and 41% homology with those of mouse, human and chicken, respectively. The protein contains tandem internal motifs array (-FN III-Ig C2-FN III- Ig C2-) in the C-terminal region which resembles to the immunoglobulin superfamily C2 and fibronectin type III motifs. The amino acid sequence of the C-terminal Ig C2 was highly conserved among MyBPs family and other thick filament binding proteins, suggesting that the C-terminal Ig C2 might play an important role in its function. All proteins belonging to MyBP-H member contains `RKPS` sequence which is assumed to be cAMP- and cGMP-dependent protein kinase A phosphorylation site. Computer analysis of the primary sequence of rat MyBP-H predicted 11 protein kinase C (PKC)phosphorylation site, 7 casein kinase II (CK2) phosphorylation site and 4N-myristoylation site.
|
|
KEYWORD
|
|
Myosin binding protein H(MyBP-H), Immunoglobulin superfamily C2, Fibronectin type III
|
|
FullTexts / Linksout information
|
|
|
|
Listed journal information
|
|
|